Control:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Recombinant:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Knock-Out:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Single Cell:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Sample Prep:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Reverse Transcription:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
Enzyme-linked Immunosorbent Assay:Article Title: Altered hepatic metabolism in Down syndrome.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Lyve1 antibody Abcam Cat# ab218535; RRID:AB_2927473 Albumin antibody Invitrogen Cat# MA5-32531, RRID:AB_2809808 Desmin antibody Abcam Cat# ab32362, RRID:AB_731901 DAPI Akoya Biosciences Cat# FP1490 PDPN antibody Abcam Cat# ab256559, RRID:AB_2936436 Smooth Muscle Actin antibody CST Cat# 19245S; RRID:AB_2734735 Anti-human Albumin antibody Proteintech Cat# 16475-1-AP; RRID:AB_2242567 Isotype control Invitrogen Cat# 31235; RRID:AB_243593 Goat anti-Rabbit IgG, Alexa Fluor TM 488 Invitrogen Cat# A27034; RRID:AB_2536097 Hoechst 33342 Abcam Cat# ab228551 Chemicals, peptides, and recombinant proteins 10x PBS, pH 7.2 Rockland Cat# MB-008 Bovine Serum Albumin Research Products International Cat# 9048-46-8 Ethylenediaminetetraacetic acid, 0.5M aq. soln, pH 8.0 Thermo scientific Cat# J62786.AK DMEM Corning Cat# 10-017-CM Collagenase P Sigma Aldrich Cat# 11213857001 Deoxyribonuclease I Worthington-Biochemical Cat# LS002145 Lysing buffer BD Biosciences Cat# 555899 Matrigel coated plates Corning Cat# 354277 mTeSR Plus media StemCell Technologies Cat# 100-0276 Accutase Fisher Scientific Cat# NC9464543 Y-27632 StemCell Technologies Cat# 72304 DMEM/F12 Cytiva Cat# SH30023.01 KnockOut Serum Replacement Gibco Cat# 11140050 1% Dimethyl-sulfoxide Sigma Aldrich Cat# D2650 Hepatocyte Growth Factor Peprotech Cat# 100-398H Dexamethasone Sigma Aldrich Cat# D4902 10x DPBS Gibco Cat# 14200-075 2-Mercaptoethanol Sigma Aldrich Cat #M6250 Syber Select Applied Biosystems Cat# 4472942 Oil Red O Alfa Aesar Cat# A12989 GlutaMAX Gibco Cat# 35050061 Non-Essential Amino Acids Gibco Cat# 11140050 Critical commercial assays Vacutainer EDTA tubes BD Cat# 36643 Allprep DNA/RNA/miRNA Universal Kit Qiagen Cat# 80224 STEMDiff Definitive Endoderm Kit StemCell Technologies Cat# 05110 Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit 10x Genomics Cat# 1000414 Rneasy Kit Qiagen Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368813 Human Albumin ELISA Kit Invitrogen Cat# EHALB (Continued on next page) Cell Reports 45, 116835, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Urea Assay Kit Abcam Cat# ab83362 Periodic Acid-Schiff Stain Sigma Aldrich Cat# 395B-1KT Deposited data HTP Sample/Participant metadata and co-occurring conditions Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488784 HTP plasma proteomics data Galbraith et al. 6 Synapse: https://doi.org/10.7303/syn31488781 Mouse liver RNA-seq data This study GEO: GSE296748 Mouse metabolomics data This study Metabolomics Workbench: ST004412, ST004413, ST004414 Experimental models: Organisms/strains B6.129S7-Dp(16Lipi-Zbtb21)1Yey/J The Jackson Laboratory Cat# JAX:013530, RRID:IMSR_JAX013530 Oligonucleotides Albumin forward: 5 ′ -GAGACCAGAGGTTGATGTGATG-3 ′ This paper – Albumin reverse: 5 ′ -AGTTCCGGGGCATAAAAGTAAG-3 ′ This paper – CYP3A4 forward: 5 ′ -AAGTCGCCTCGAAGATACACA -3 ′ This paper – CYP3A4 reverse: 5 ′ -AAGGAGAGAACACTGCTCGTG-3’.
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